Coding

Part:BBa_J51000:Design

Designed by: Peter Nguyen   Group: iGEM2006_Rice   (2006-09-06)


ComP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 927
    Illegal NotI site found at 1899
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: The Biobrick prefix and suffix sites are non-standard! Due to the high percentage of A+Ts in the ComQXPA genes, the primer hybridization sites were lengthened and the Biobrick sites correspondingly shortened (the NotI sites between the E-X and S-P restriction sites was removed).


Primers used for isolation and construction (5'->3')

F: cggaattccctctagATGAAGAACTTAATAAAAAAATTCACAATAGCTG

R: gactgcagctactagTTACAATTCGATTTCAATATCAGCCTTAAAGCC

NOTE: The ComP part contains an internal SpeI site, which prevents cloning using standard assembly. This will be removed by site-directed mutagenesis in the future.

Source

Bacillus subtilis 168 genomic

References